Mouse Anti Human CD4, IgG2a, Clone: [Edu-2], Monoclonal

Product Overview

• Article Name: Mouse Anti Human CD4, IgG2a, Clone: [Edu-2], Monoclonal
• Biozol Catalog Number: NMB-MUB3002P1
• Supplier Catalog Number: MUB3002P1
• Alternative Catalog Number: NMB-MUB3002P1
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC, ICC, IHC-Fr, IHC-P
• Species Reactivity: Human

Product Description

CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [Edu-2]
• Isotype: IgG2a
• UniProt: P01730
• Buffer: Each vial contains 1 ml of 100µg/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.
• Source: Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.
• Formula: Each vial contains 1 ml of 100µg/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.

Application Notes

• Application Notes: Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 µl/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 µl of the purified monoclonal antibody with RaM FITC conjugate and 100 µl blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.