The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double
Clonality:
Polyclonal
Clone Designation:
[Polyclonal]
Buffer:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit se
Source:
Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formula:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit se
Antibody Type:
Secondary Antibody
Application Notes:
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and
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