Sheep anti Human IgG4 (subclass specific), Clone: [Polyclonal], Monoclonal
Biozol Catalog Number:
NMB-SHAHU/IGG4
Supplier Catalog Number:
ShAHu/IgG4
Alternative Catalog Number:
NMB-SHAHU/IGG4
Manufacturer:
NordicMubio
Host:
Sheep
Category:
Antikörper
Species Reactivity:
Human
Conjugation:
Unconjugated
Alternative Names:
Sheep anti Human IgG4 (subclass specific), Clone: [Polyclonal], Monoclonal
Format:
Antiserum
Target Specificity:
IgG4 (Fc)
Cross-Adsorption (MinX):
no cross-adsorbtion
The activity of the antiserum is directed to human IgG4 subclass. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and dou
Clonality:
Monoclonal
Clone Designation:
[Polyclonal]
Buffer:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep seru
Source:
Purified homogenous IgG4 is isolated from human serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formula:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep ser
Antibody Type:
Secondary Antibody
Application Notes:
Precipitation assays. Because of their characteristics and those of the subclass-specific antigenic determinants, the use of this antiserum requires special precautions. The specificity and reactivity depend largely on the conditions of the test system in which they are applied. The performance of NORDIC polyclonal subclass-specific antisera is guaranteed when they are used for the purposes described in RWP 5E/88, applying the special immunoprecipitation arrangement recommended for these purposes. In immunoelectrophoresis use 2 µl 1:8, 1:16 and 1:32 dilutions of pathological serum or equivalent against 60 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Nordic also makes available a human standard serum with assigned values of the subclasses of IgG (NOR-01). The antigenic differences between subclasses are considerably less pronounced than those between classes or between light chain types. Subclass-specific determinants are only few in number and probably restricted in their structural exposure, which makes them relative weak immunogens. This explains why the titres of subclass-specific antisera are usually lower than those of class-specific antisera which react with many antigenic determinants commonly present on all molecules of the respective class.
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