Sheep anti Rat IgG2b (subclass specific), Clone: [Polyclonal], Monoclonal
Format:
Antiserum
Target Specificity:
IgG2b (Fc)
Cross-Adsorption (MinX):
no cross-adsorbtion
The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial i
Clonality:
Polyclonal
Clone Designation:
[Polyclonal]
Buffer:
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added as it may interfere with he antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign protein
Source:
Pools of purified homogenous IgG2b isolated from pooled rat serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formula:
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added as it may interfere with he antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign protein
Antibody Type:
Secondary Antibody
Application Notes:
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and
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