The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H]
Application Notes:
1. 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips 2. Wash bottle or multichannel dispenser for washing 3. 2000 ml graduated cylinder 4. PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4) 5. Deionized or distilled H2O 6. Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450-540 or 450-595 nm or a single read at 450 nm. 7. Tissue culture microtiter plate (96 well culture dish) 8. Sterile reagent troughs 9. Micro syringe filter (0.2 µm) 10. Syringe
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