Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membr
Concentration:
See vial for concentration
Buffer:
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline
Formula:
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline
Application Notes:
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.
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