A portion of amino acids 47-76 from human CH25H was used as the immunogen for the CH25H antibody.
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. [UniProt]
The stated application concentrations are suggested starting points. Titration of the CH25H antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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