A portion of amino acids 830-860 from the human protein were used as the immunogen for the NOS2 antibody.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:25-1:50,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Application Notes:
The stated application concentrations are suggested starting points. Titration of the NOS2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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