A portion of amino acids 66-94 from the human protein were used as the immunogen for the LH1 antibody.
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Western blot: 1:500-1:1000,Immunohistochemistry (FFPE): 1:10-1:5,Flow cytometry: 1:25 (1x10e6 cells)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the LH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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