A portion of amino acids 236-264 from the human protein were used as the immunogen for the IDUA antibody.
IDUA encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans.
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:50-1:100,Flow cytometry: 1:25 (1x10e6 cells)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the IDUA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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