A portion of amino acids 246-274 from the human protein were used as the immunogen for the GCLM antibody.
GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:25-50,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Application Notes:
The stated application concentrations are suggested starting points. Titration of the GCLM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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