E.coli-derived mouse IFN Gamma/Ifng recombinant protein (Position: H23-C155) was used as the immunogen for the Ifng antibody.
IFNG antibody detects Interferon gamma, encoded by the IFNG gene on chromosome 12q15. IFNG antibody is widely used in immunology research to study cytokine biology, host defense, and inflammation. Interferon gamma (IFN g) is a type II interferon produced primarily by activated T lymphocytes and natural killer (NK) cells. It functions as a pleiotropic cytokine coordinating innate and adaptive immune responses. IFN g induces expression of hundreds of genes involved in antigen presentation, macrophage activation, and antiviral defense.Structurally, IFN g is a homodimeric glycoprotein of ~17 kDa per subunit, belonging to the class II cytokine family. It signals through the interferon gamma receptor (IFNGR1/IFNGR2 complex), activating the JAK-STAT pathway. STAT1 phosphorylation leads to transcription of interferon-stimulated genes that mediate antimicrobial and immune regulatory functions.Functionally, IFN g activates macrophages to enhance phagocytosis and production of reactive oxygen/nitrogen species, boosts antigen presentation by inducing MHC class I and II, and shapes adaptive immunity by promoting Th1 differentiation. It also enhances NK and cytotoxic T cell activity. IFN g deficiency results in severe susceptibility to mycobacterial and viral infections. Researchers use IFNG antibody to study cytokine signaling, immune activation, and host defense mechanisms.Clinically, IFN g is implicated in infection, autoimmunity, and cancer. Mutations in IFNG or its receptor cause Mendelian susceptibility to mycobacterial disease. Elevated IFN g contributes to autoimmunity, including rheumatoid arthritis and lupus, where it drives chronic inflammation. IFN g also has roles in tumor immunology, promoting antigen presentation but sometimes contributing to immune evasion. Recombinant IFN g is used therapeutically for chronic granulomatous disease and osteopetrosis. NSJ Bioreagents supplies IFNG antibody for immunology, infectious disease, and oncology research.Experimentally, IFNG antibody is used in ELISA to quantify secreted cytokine, in western blotting to detect the ~17 kDa monomer, in immunohistochemistry to assess tissue cytokine levels, and in flow cytometry to measure intracellular production in T cells and NK cells.
