| RAD51AP1 antibody detects RAD51-associated protein 1, encoded by the RAD51AP1 gene on chromosome 12p13.33. RAD51AP1 antibody is widely applied in DNA repair research, genomic stability studies, and cancer biology. RAD51AP1 functions as a binding partner of RAD51, the key recombinase in homologous recombination repair of double-strand DNA breaks. By stimulating RAD51-mediated strand invasion and exchange, RAD51AP1 supports accurate DNA repair and maintains chromosomal integrity.Structurally, RAD51AP1 is a ~42 kDa protein containing a central RAD51-interacting domain and regions that promote DNA binding. It associates with single-stranded and double-stranded DNA, enhancing RAD51 filament stability. RAD51AP1 forms nuclear foci at DNA damage sites, co-localizing with repair proteins such as BRCA2 and RPA. Alternative splicing generates isoforms with different activities and localizations.Functionally, RAD51AP1 is a positive regulator of homologous recombination. It enhances RAD51-mediated D-loop formation, critical for strand invasion during repair. Loss of RAD51AP1 reduces repair efficiency, increases chromosomal instability, and sensitizes cells to DNA-damaging agents. Overexpression can protect cells from genotoxic stress, but in cancer it may promote resistance to chemotherapy. Researchers use RAD51AP1 antibody to study DNA repair, homologous recombination, and cancer resistance mechanisms.Clinically, RAD51AP1 is implicated in cancer susceptibility and therapy resistance. Its overexpression is observed in breast, ovarian, and colon cancers, correlating with poor prognosis. As a RAD51 cofactor, it represents a potential therapeutic target for sensitizing tumors to DNA-damaging agents such as platinum drugs or PARP inhibitors. RAD51AP1 polymorphisms may also influence genetic predisposition to cancer. NSJ Bioreagents supplies RAD51AP1 antibody for oncology, DNA repair, and therapeutic research.Experimentally, RAD51AP1 antibody is used in western blotting to detect the ~42 kDa protein, in immunofluorescence microscopy to visualize nuclear foci after DNA damage, and in immunohistochemistry to assess tumor expression. Co-immunoprecipitation with RAD51AP1 antibody identifies complexes with RAD51, BRCA2, and other recombination proteins. |
