The amino acid sequence used as immunogen for this antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.
Acid phosphatases are a broad family of enzymes that differ widely in their amino acid length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid Phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Formula:
1X PBS, pH 7.4
Target:
Acid Phosphatase
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1-3ug/ml
Application Notes:
Provided assay concentrations are suggestions only, Acid Phosphatase antibody titration may be required for optimal results. 1. This antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase.
Western blot testing of human samples using Acid Phosphatase antibody at 1ug/ml.
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