A recombinant protein fragment from the N-terminal portion of Acid Phosphatase / ACP1 was used as the immunogen for this antibody. This AA sequence is common to the A, C and D isoforms.
Acid phosphatases (AP) are a broad family of enzymes that differ widely in their AA length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Formula:
1X PBS, pH 7.4
Target:
Acid Phosphatase / ACP1
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1-3ug/ml
Application Notes:
Provided assay concentrations are suggestions only, ACP1 antibody titration may be required for optimal results. 1. This antibody will detect the A, C and D isoforms of Acid Phosphatase.
Western blot testing of human samples with Acid Phosphatase / ACP1 antibody at 1ug/ml.
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