A recombinant protein fragment from the internal region of 14-3-3 sigma was used as the immunogen for this antibody. The amino acid sequence is common to the ~30kDa and ~20kDa isoforms.
14-3-3 proteins are regulators of a diverse group of signaling proteins, including kinases, phosphatases, and transmembrane receptors, and have roles in a wide range of physiological processes including transcription, intracellular signaling, cell cycle control and apoptosis. There are seven 14-3-3 proteins in most mammals: beta, gamma epsilon, zeta, eta, sigma and tau. 14-3-3 sigma, also called Stratifin, is a product of the SFN gene. It is a tumor suppressor gene inactivated via DNA methylation. Reduced or no expression of 14-3-3 sigma is seen in various carcinoma including skin, breast and prostate, suggesting that 14-3-3 gene silencing may be involved in tumor progression.
