An E.coli-derived zebrafish Kv1.2/Kcna2a recombinant protein (amino acids E437-V493) was used as the immunogen for the Zebrafish Kv1.2 antibody.
The Kv1.2 protein in zebrafish is a voltage-gated potassium channel subunit encoded by the kcna2a and kcna2b genes, which are paralogs resulting from the teleost-specific whole genome duplication. Kv1.2 belongs to the Shaker-related (Kv1) family of potassium channels, which play critical roles in regulating membrane excitability in neurons and other excitable cells.Kv1.2 forms tetrameric channel complexes, either as homotetramers or heterotetramers with other Kv1 subunits, to mediate delayed rectifier potassium currents. These channels open in response to membrane depolarization, allowing K+ ions to flow out of the cell, thereby repolarizing the membrane and shaping action potentials.In zebrafish, kcna2a and kcna2b are expressed in the central nervous system (CNS), particularly during early development, suggesting roles in neuronal differentiation, patterning, and excitability. Functional studies in zebrafish have shown that perturbations in Kv1.2 expression or function can lead to abnormal neural activity and motor behaviors, highlighting its importance in the maturation and function of the nervous system.Kv1.2 is also a target for pharmacological research, as dysfunction in Kv1.2 channels in humans is associated with disorders such as episodic ataxia, epilepsy, and neuromyotonia. Zebrafish models expressing human Kv1.2 variants or subjected to gene knockdowns provide valuable platforms for studying disease mechanisms and for high-throughput drug screening.
