An E.coli-derived zebrafish Psmc4 recombinant protein (amino acids M1-D410) was used as the immunogen for the Zebrafish Psmc4 antibody.
PSMC4 (Proteasome 26S Subunit, ATPase 4), also known as Rpt3, is an essential component of the 19S regulatory particle of the 26S proteasome, a multi-subunit complex responsible for ATP-dependent degradation of ubiquitinated proteins. In zebrafish (Danio rerio), Psmc4 is highly conserved and serves a critical role in maintaining cellular protein homeostasis by regulating the degradation of misfolded, damaged, or regulatory proteins.PSMC4 is one of six AAA+ ATPases (Rpt1a€''Rpt6) that form a hexameric ring within the base of the 19S proteasome regulatory particle. This ATPase activity is required for substrate unfolding and translocation into the 20S core particle for proteolysis. In zebrafish, psmc4 is expressed ubiquitously, with elevated expression in rapidly dividing and metabolically active tissues, underscoring its involvement in cell cycle regulation, signal transduction, and stress responses.Functional analyses in zebrafish models have demonstrated that disruption of psmc4 leads to proteasome dysfunction, accumulation of ubiquitinated proteins, and developmental abnormalities. Because of its central role in protein degradation, zebrafish PSMC4 is a valuable target for studying proteostasis, neurodegenerative disease mechanisms, and the effects of proteasome inhibitors in vivo.
