CD20 Antibody PE Conjugate, Clone: [B9E9], Mouse, Monoclonal

Product Overview

• Article Name: CD20 Antibody PE Conjugate, Clone: [B9E9], Mouse, Monoclonal
• Biozol Catalog Number: NSJ-V2048PE-100T
• Supplier Catalog Number: V2048PE-100T
• Alternative Catalog Number: NSJ-V2048PE-100T
• Manufacturer: NSJ Bioreagents
• Host: Mouse
• Category: Antikörper
• Application: FACS, IF
• Species Reactivity: Human
• Immunogen: The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.
• Conjugation: PE

Product Description

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

Product Properties

• Clonality: Monoclonal
• Concentration: 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Clone Designation: [B9E9]
• Isotype: Mouse IgG2a, kappa
• NCBI: 931
• Buffer: 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Form: 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Target: CD20
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence (1:50-1:100)
• Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.