Human eosinophils from a patient with hypereosinophilic syndrome were used as the immunogen for this Eosinophil peroxidase antibody.
Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
Clonality:
Monoclonal
Concentration:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Purity:
Protein G purified monoclonal antibody
Form:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Target:
Eosinophil Peroxidase
Antibody Type:
Primary Antibody
Application Dilute:
ELISA (order BSA/sodium azide-free format for coating),Immunofluorescence: 1-3ug/ml,Flow cytometry: 1-2ug/million cells
Application Notes:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. <a href=http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.1993.tb05948.x/epdf>EPO detection by ELISA using mAb AHE-1
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