Human tonsil B cells (L26) and recombinant protein (IGEL/733) were used as the immunogen for this CD20 antibody cocktail.
This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
Flow cytometry: 5-10ul/10 6 cells,Immunofluorescence: 1:50-1:100,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT
Application Notes:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody cocktail to be titered up or down for optimal performance.
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