Cytochrome C Antibody, Clone: [CYCS/1010], Mouse, Monoclonal
Biozol Catalog Number:
NSJ-V2789-20UG
Supplier Catalog Number:
V2789-20UG
Alternative Catalog Number:
NSJ-V2789-20UG
Manufacturer:
NSJ Bioreagents
Host:
Mouse
Category:
Antikörper
Application:
IHC-P, WB
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for the Cytochrome C antibody.
Cytochrome C, or CYCS, is an electron carrier protein. The oxidized form of the CYCS heme group can accept an electron from the heme group of the Cytochrome C1 subunit of cytochrome reductase. CYCS then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. CYCS also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of CYCS into the cytosol. Binding to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt]
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the Cytochrome C antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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