Blood Group Lewis a Antibody, Clone: [7LE], Mouse, Monoclonal
Biozol Catalog Number:
NSJ-V3058-100UG
Supplier Catalog Number:
V3058-100UG
Alternative Catalog Number:
NSJ-V3058-100UG
Manufacturer:
NSJ Bioreagents
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF, IHC-P
Species Reactivity:
Human, Mouse
Immunogen:
Mucins isolated from ovarian cyst fluid were used as the immunogen for the Blood Group Lewis a antibody.
Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Clonality:
Monoclonal
Clone Designation:
[7LE]
UniProt:
Not Applicable
Purity:
Protein G affinity chromatography
Form:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Flow cytometry: 0.5-1ug/10 6 cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the Blood Group Lewis a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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