TIMP-2 Antibody, Clone: [3A4], Mouse, Monoclonal

Product Overview

• Article Name: TIMP-2 Antibody, Clone: [3A4], Mouse, Monoclonal
• Biozol Catalog Number: NSJ-V3344SAF-100UG
• Supplier Catalog Number: V3344SAF-100UG
• Alternative Catalog Number: NSJ-V3344SAF-100UG
• Manufacturer: NSJ Bioreagents
• Host: Mouse
• Category: Antikörper
• Application: FACS, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Amino acids 56-66 (DSGNDIYGNPI) from the human protein were used as the immunogen for this TIMP-2 antibody.

Product Description

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [3A4]
• UniProt: P16035
• Buffer: 1X PBS, pH 7.4
• Purity: Protein G affinity chromatography
• Form: 1 mg/ml in 1X PBS, BSA free, sodium azide free
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow cytometry: 0.5-1ug/10 6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.