Western blot: 0.5-1ug/ml,Flow cytometry: 1-2ug/10 6 cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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