Full length protein was used as the immunogen for this CCNA2 antibody.
This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.
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