A portion of amino acids 29-107 from the human protein was used as the immunogen for the CD40 antibody.
Cluster of differentiation 40, CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. [Wiki]
Flow cytometry: 1-2ug/10 6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
The stated application concentrations are suggested starting points. Titration of the CD40 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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