A DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase was used as the immunogen for the DNA-RNA Hybrid antibody.
We have not tested this antibody in-house in Immunofluorescence, CHIP, Immunocytochemistry, Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone.DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Clonality:
Monoclonal
Clone Designation:
[S9.6]
Purity:
Protein G affinity
Form:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Antibody Type:
Primary Antibody
Application Dilute:
Chromatin immunoprecipitation: per published data,Flow cytometry: per published data,Immunocytochemistry: per published data,Immunofluorescence: per published data
Application Notes:
Optimal dilution of the DNA-RNA Hybrid antibody should be determined by the researcher.
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