Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.
Clonality:
Monoclonal
Concentration:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Purity:
Protein G affinity chromatography
Form:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Target:
p27
Antibody Type:
Primary Antibody
Application Dilute:
Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Notes:
Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
IHC testing of FFPE hu
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