Smooth Muscle Actin Antibody, Clone: [SMAT2-1], Mouse, Monoclonal

Product Overview

• Article Name: Smooth Muscle Actin Antibody, Clone: [SMAT2-1], Mouse, Monoclonal
• Biozol Catalog Number: NSJ-V7150SAF-100UG
• Supplier Catalog Number: V7150SAF-100UG
• Alternative Catalog Number: NSJ-V7150SAF-100UG
• Manufacturer: NSJ Bioreagents
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-P
• Species Reactivity: Human, Rat
• Immunogen: Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.

Product Description

Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml in 1X PBS, BSA free, sodium azide free
• Clone Designation: [SMAT2-1]
• Isotype: Mouse IgG2a, kappa
• UniProt: P62736
• Buffer: 1 mg/ml in 1X PBS, BSA free, sodium azide free
• Purity: Protein G affinity chromatography
• Form: 1 mg/ml in 1X PBS, BSA free, sodium azide free
• Target: Alpha Actin
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application Notes: Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Images

IHC testing