A recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody.
This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/10 6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
* VAT and and shipping costs not included. Errors and price changes excepted
