RBL-2H3 cells expressing human CLEC9A fused to an HA epitope. Its epitope maps to within amino acids 50-110.
This MAb recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor.
Clonality:
Monoclonal
Concentration:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Clone Designation:
[8F9]
Isotype:
Mouse IgG2a, kappa
UniProt:
Q8RU4
Buffer:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Purity:
Protein G affinity chromatography
Form:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Target:
CLEC9A
Antibody Type:
Primary Antibody
Application Dilute:
Flow cytometry: 1-2ug/10 6 cells in 0.1ml,Immunofluorescence: 1-2ug/ml
Application Notes:
Optimal dilution of the CLEC9A antibody should be determined by the researcher.
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