Anti-Multi-Cytokeratin Antibody, Clone: [SPM583], Mouse, Monoclonal

Catalog Number: NSJ-V9112IHC-7ML
Article Name: Anti-Multi-Cytokeratin Antibody, Clone: [SPM583], Mouse, Monoclonal
Biozol Catalog Number: NSJ-V9112IHC-7ML
Supplier Catalog Number: V9112IHC-7ML
Alternative Catalog Number: NSJ-V9112IHC-7ML
Manufacturer: NSJ Bioreagents
Host: Mouse
Category: Antikörper
Application: IHC-P
Species Reactivity: Human
Immunogen: A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.
This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Clonality: Monoclonal
Clone Designation: [SPM583]
UniProt: P19013
Purity: Protein G affinity chromatography
Form: Prediluted in 1X PBS, 0.1 mg/ml BSA (US sourced), 0.05% sodium azide, *For IHC use only*
Antibody Type: Primary Antibody
Application Dilute: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Notes: The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.