Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality:
Monoclonal
Clone Designation:
[SPM375]
UniProt:
Not Applicable
Purity:
Protein G affinity chromatography
Form:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Antibody Type:
Primary Antibody
Application Dilute:
Flow cytometry: 1-2ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
* VAT and and shipping costs not included. Errors and price changes excepted
