Muscle Specific Actin Antibody, Clone: [SPM160], Mouse, Monoclonal
Biozol Catalog Number:
NSJ-V9114-20UG
Supplier Catalog Number:
V9114-20UG
Alternative Catalog Number:
NSJ-V9114-20UG
Manufacturer:
NSJ Bioreagents
Host:
Mouse
Category:
Antikörper
Application:
IHC-P
Species Reactivity:
Human
Immunogen:
Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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