SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a], Unconjugated, Rabbit, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a], Unconjugated, Rabbit, Monoclonal
• Biozol Catalog Number: PRS-10-750
• Supplier Catalog Number: 10-750
• Alternative Catalog Number: PRS-10-750-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Virus
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P001A]: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: batch dependent
• Clone Designation: [75G5a]
• NCBI: 43740575
• Buffer: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: Paired with 84C4a or 84D7, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).

Images

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 75G5a wasserially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and85C10-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C10-biotin detectedCOVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and85C1-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C1-biotin detectedCOVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg).

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-750, 1 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal Antibody (75G5a), CoV2NP-75G5.