GRP78 / BiP Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GRP78 / BiP Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-101
• Supplier Catalog Number: 13-101
• Alternative Catalog Number: PRS-13-101-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1).
• Conjugation: Unconjugated
• Alternative Names: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 78kDa
• NCBI: 3309
• UniProt: P11021
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.