CD4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CD4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-152
• Supplier Catalog Number: 13-152
• Alternative Catalog Number: PRS-13-152-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1).
• Conjugation: Unconjugated
• Alternative Names: CD4 Antibody [9H5A8] : CD4mut

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 51kDa
• NCBI: 920
• UniProt: P01730
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of U-937 cells, using CD4 antibody (13-152) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody (13-152) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.