CDK4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CDK4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-154
• Supplier Catalog Number: 13-154
• Alternative Catalog Number: PRS-13-154-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CDK4 (NP_000066.1).
• Conjugation: Unconjugated
• Alternative Names: CDK4, CMM3, PSK-J3

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 34kDa
• NCBI: 1019
• UniProt: P11802
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using CDK4 antibody (13-154). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.