DES Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-255
Article Name: DES Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-255
Supplier Catalog Number: 13-255
Alternative Catalog Number: PRS-13-255-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 121-470 of human DES (NP_001918.3).
Conjugation: Unconjugated
Alternative Names: DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, LGMD2R
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 54kDa
NCBI: 1674
UniProt: P17661
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of mouse heart, using DES antibody (13-255).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human appendix using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.