PTCH1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PTCH1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-291
• Supplier Catalog Number: 13-291
• Alternative Catalog Number: PRS-13-291-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).
• Conjugation: Unconjugated
• Alternative Names: PTCH1, PTC, BCNS, HPE7, PTC1, PTCH, NBCCS, PTCH11

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 160kDa
• NCBI: 5727
• UniProt: Q13635
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:1000IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using PTCH1 antibody (13-291) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.