YAP1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: YAP1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-379
• Supplier Catalog Number: 13-379
• Alternative Catalog Number: PRS-13-379-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
• Conjugation: Unconjugated
• Alternative Names: YAP1, YAP, YAP2, YAP65, YKI

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 72kDa
• NCBI: 10413
• UniProt: P46937
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using YAP1 antibody (13-379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody (13-379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using YAP1 antibody (13-379) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YAP1 antibody (13-370) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using YAP1 antibody (13-379) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug YAP1 antibody (13-379). Western blot was performed from the immunoprecipitate using YAP1 antibody (13-379) at a dilition of 1:1000.