ROCK1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ROCK1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-417
• Supplier Catalog Number: 13-417
• Alternative Catalog Number: PRS-13-417-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
• Conjugation: Unconjugated
• Alternative Names: ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 158kDa
• NCBI: 6093
• UniProt: Q13464
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (13-417) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (13-417) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody (13-417) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody (13-417) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody (13-417) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (13-417). Western blot was performed from the immunoprecipitate using ROCK1 antibody (13-417) at a dilition of 1:1000.