EGLN1/EGLN2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-591
Article Name: EGLN1/EGLN2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-591
Supplier Catalog Number: 13-591
Alternative Catalog Number: PRS-13-591-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide of human EGLN1/EGLN2
Conjugation: Unconjugated
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 44kDa
NCBI: 54583
UniProt: Q9GZT9
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody (13-591) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).