POLR2A Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: POLR2A Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-946
• Supplier Catalog Number: 13-946
• Alternative Catalog Number: PRS-13-946-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
• Conjugation: Unconjugated
• Alternative Names: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 250kDa
• NCBI: 5430
• UniProt: P24928
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using POLR2A antibody (13-946) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse pancreas using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).