FAK Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: FAK Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-952
• Supplier Catalog Number: 13-952
• Alternative Catalog Number: PRS-13-952-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (NP_722560.1).
• Conjugation: Unconjugated
• Alternative Names: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 120kDa
• NCBI: 5747
• UniProt: Q05397
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using PTK2 antibody (13-952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human colon using FAK antibody (13-952) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using FAK antibody (13-952) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug FAK antibody (13-952). Western blot was performed from the immunoprecipitate using FAK antibody (13-952) at a dilition of 1:1000.