SIRT1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SIRT1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-968
• Supplier Catalog Number: 13-968
• Alternative Catalog Number: PRS-13-968-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
• Conjugation: Unconjugated
• Alternative Names: SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 140kDa
• NCBI: 23411
• UniProt: Q96EB6
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using SIRT1 antibody (13-968) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (13-968) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody (13-968) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using SIRT1 antibody (13-968) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SIRT1 antibody (13-968) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (13-968). Western blot was performed from the immunoprecipitate using SIRT1 antibody (13-968) at a dilition of 1:1000.