EGFR Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: EGFR Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-055
• Supplier Catalog Number: 14-055
• Alternative Catalog Number: PRS-14-055-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
• Conjugation: Unconjugated
• Alternative Names: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 175kDa
• NCBI: 1956
• UniProt: P00533
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody (14-055) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (14-055) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Immunofluorescence analysis of HeLa cells using EGFR antibody (14-055) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (14-055) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using EGFR antibody (14-055) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug EGFR antibody (14-055). Western blot was performed from the immunoprecipitate using EGFR antibody (14-055) at a dilition of 1:1000.