HNRNPL Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-428
Article Name: HNRNPL Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-428
Supplier Catalog Number: 14-428
Alternative Catalog Number: PRS-14-428-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human HNRNPL (NP_001524.2).
Conjugation: Unconjugated
Alternative Names: HNRPL, HNRPL, hnRNP-L, P/OKcl.14
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 64kDa
NCBI: 3191
UniProt: P14866
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HNRNPL antibody (14-428) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.