EP300 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: EP300 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-746
• Supplier Catalog Number: 14-746
• Alternative Catalog Number: PRS-14-746-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2).
• Conjugation: Unconjugated
• Alternative Names: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 280kDa
• NCBI: 2033
• UniProt: Q09472
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using EP300 antibody (14-746) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using EP300 antibody (14-746) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (14-746) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using EP300 antibody (14-746) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).